Physiological analyses including relative leaf water content, electrolyte leakage, proline content, malondialdehyde (MDA) content, H2O2 content and sodium and potassium accumulation indicated that the OsDST-SRDX fusion gene enhanced salt tolerance in transgenic perennial ryegrass by altering a wide range of physiological responses. Transgenic lines overexpressing the OsDST-SRDX fusion gene showed obvious phenotypic differences and clear resistance to salt-shock and to continuous salt stresses compared to non-transgenic plants. Integration and expression of the OsDST-SRDX in transgenic plants were tested by PCR and RT-PCR, respectively. Here, the rice DST gene was linked to an SRDX domain for gene expression repression based on the Chimeric REpressor gene-Silencing Technology (CRES-T) to make a chimeric gene (OsDST-SRDX) construct and introduced into perennial ryegrass by Agrobacterium-mediated transformation. Phylogenetic analysis of six homologues of DST genes in different plant species revealed that DST genes were conserved evolutionarily. 1984 53:293–321.The Drought and Salt Tolerance gene (DST) encodes a C2H2 zinc finger transcription factor, which negatively regulates salt tolerance in rice (Oryza sativa). Evidence for a repeating domain in type I restriction enzymes. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. A comprehensive set of sequence analysis programs for the VAX. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Sanger F, Coulson AR, Barrell BG, Smith AJ, Roe BA.The making of strand-specific M13 probes. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. A hybrid recognition sequence in a recombinant restriction enzyme and the evolution of DNA sequence specificity. Purification and DNA recognition sequences. Two type I restriction enzymes from Salmonella species. Nagaraja V, Shepherd JC, Pripfl T, Bickle TA.The nucleotide sequence recognised by the Escherichia coli D type I restriction and modification enzyme. Nagaraja V, Stieger M, Nager C, Hadi SM, Bickle TA.Genetic recombination can generate altered restriction specificity. Fuller-Pace FV, Bullas LR, Delius H, Murray NE.SQ, a new system derived by recombination between the SB system of Salmonella typhimurium and the SP system of Salmonella potsdam. DNA restriction and modification systems in Salmonella. Sequence diversity among related genes for recognition of specific targets in DNA molecules. Genetic complementation between the K and B systems of Escherichia coli and the Salmonella typhimurium system SB, with the same chromosomal location. Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli. A complementation analysis of the restriction and modification of DNA in Escherichia coli. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. typhimurium and Escherichia coli K-12 are identical, and the 5' segments of their hsdS genes are strikingly homologous rather than variable. Furthermore, the trinucleotide components of the recognition sequences of S. Our analysis implicates at least parts of the variable regions in the determination of the specificity of interaction between protein and DNA. A polypeptide domain encoded on the 5' side of the crossover dictates recognition of the trinucleotide component of the target sequence, and a second domain, encoded on the 3' side of the crossover, similarly governs recognition of the tetra- or penta-nucleotide component. Concomitant with the generation of a new combination of flanking variable regions is the recombination of minor differences in the central conserved region. We have localized the position of the crossover in the central conserved region by analysis of nucleotide sequences. Recombination between the hsdS genes of SB and SP generated a system (SQ) with a different recognition specificity. The hsdS genes of the SB and SP systems have a conserved sequence of around 100 base pairs flanked by two nonhomologous (variable) regions of around 500 base pairs. The hsd genes of Salmonella typhimurium and Salmonella potsdam encode related type I restriction and modification systems designated SB and SP, respectively the polypeptide encoded by the hsdS gene dictates the DNA sequence recognized.
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